I have a dataset which contains monthly sampled zooplankton data. It is always for the same sampling site so not spatial, but over 10 years. I wanted to use an NMDS plot to see if the zooplankton community (during MArch, April and May) from 2014-2024 would be grouped into distinct communities using an NMDS but it comes out arch shaped. Can I solve this? The samples do not have extra environmental variables. I used the total abundance (m-3) of each sample.
nmds <- metaMDS(dist_matrix, k = 2, trymax = 100)
stress_value<-nmds$stress
nmds_scores <- as.data.frame(scores(nmds))
nmds_scores$month <- Total_Abundance_Spring_Mero$Month
nmds_scores$year <- Total_Abundance_Spring_Mero$Year
# Plot NMDS
ggplot(nmds_scores, aes(x = NMDS1, y = NMDS2, color = factor(year), shape = factor(month))) +
geom_point(size = 3) +
labs(title = "Total abundance (m-3) of Meroplankton in Spring", x = "NMDS1", y = "NMDS2", color = "Year", shape = "Month") +
theme_classic()+
annotate("text", x = Inf, y = Inf, label = paste("Stress =", round(stress_value, 4)),
hjust = 1.1, vjust = 1.1, size = 5, color = "red")````
[Arch Shaped NMDS plot, shapes represent the month and color the year](https://i.sstatic.net/4aBN7ffL.png)
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