I am working on sequences that I have to blast over nt database of NCBI using the blastn command on bash.
It worked perfectly yesterday and now decided to not work for unknown reason.
here is my command line
blastn -query Without_Primers2.fasta -db nt -remote -out Blast_res.txt -outfmt "6 qseqid qlen sseqid slen length gaps qcovs pident staxids"
When I send the command, it create a blast_res.txt file as expected but without anything in it. and the command is running endlessly on the terminal.
back when it worked, I could see the result getting saved on the txt file as the command was running but now there is nothing on it anymore.
I have basically tried everything from uninstalling to installing back and rebooting my computer but the command seems to just not work anymore. I also do not have any error message, as the command just keep running endlessly.
I really need this command to work and would appreciate any help. Thanks !
rebooting computer
deinstall and install blast
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