I am new to bioinformatics and I am not sure what the erorr could be.
I want to analyze single cell data with Seurat, I have filtered my genes.tsv file, to only contain protein coding genes, since then I can not create a seurat object when merging my files (barcode.tsv, genes.tsv, matrix.tsv)
here is how the code and output looks like

> for (sample in files){
+   tmp_data = Read10X(data.dir = paste0(working_dir,"/raw_only_protein_coding/",sample,"/longreads_genes_seurat"))
+   tmp_seu = CreateSeuratObject(counts = tmp_data,
+                                project = sample)
+   tmp_seu$sample = sample
+   tmp_seu$disease = sample_meta[sample,"disease"]
+   tmp_seu$region = sample_meta[sample,"region"]
+   assign(sample,tmp_seu)
+ }
Error in fixupDN.if.valid(value, x@Dim) : 
  length of Dimnames[[1]] (168) is not equal to Dim[1] (22517)

thank you

I made sure that the genes file did not have a different structure, added headers, only difference between original and it, is that the rows with genes with non coding protein function are deleted.