I am utilizing OpenCV and Python to located the positions of the 96-well plates in order to automate a detection process. However, I encounter an issue that the camera is builded with a wide angle lens resulting in an image which is not ideal for Hough Transform to detect and locate circles, in this case wells, as the function detects the inside of the wells as a circle. I have include a sample well-image and an image of what was detected by the wells.