I’m quite new to scripting and I’ve attempted to create a TrackMate macro using the tutorials online. My images are dark spots on a light background so I have to invert the image and I also resize the image otherwise I start to get memory errors. It seems to be successfully detecting my targets as spots, and shows multiple spots lining up on the tracks left by targets, but won’t generate the tracks themselves. There are no errors when I run the code and I’ve manually tracked everything with the GUI and used the same settings that worked there.