I’m using Fiji and I’m a beginner. I have a set of images from an experiment, put them in a stack and then do a dynamic reslice, around the middle of the stack. In the reslice I want to extract the thin line over the larger black “line” (shown by the arrow in the image) and my problem is that I can’t extract the line successfully.

I am currently working on a project that involves analyzing multiple aluminum layers, each with distinct properties. These layers are presently represented as separate layers within Fiji software. My objective is to stack all these layers to create a single volumetric model and export it as a STEP file, which I can then import into SolidWorks for finite element analysis (FEA).