The Golang application I hosted on the EC2 instance works fine and loads the env file when I build it and run it manually, but when running it as service it fails to get the environment file even though I have specified the Environment File path in the service conf too

Systemd Service Configuration (project.service

Project Directory Structure

Error Log Generated During Service Execution

FilePath used for loading .env

How can I troubleshoot why the .env file is not being loaded when the application is run as a systemd service?
Are there any configurations I should check to ensure the .env file is accessible to the systemd service?