I’m working in a project that requires the use 4 software’s. 1 call PRIME that use MixMHCpred and the other 3 netMHCpan, nectchop and netMCHstab.
I run the terminal command for each of then (I’m working in Linux) and the program’s run with no problems. But now I’m traying to run them from a python script (I’m using visual code studio) and it doesn’t work, i trait with os.system and subprocess and it doesn’t work, I’ve trait to run a bash script with just the command and it just work in the terminal, not from the python script. The only output i get is “x command doesn’t exist”, the software’s were install in the base env and using path, I’m using the python 3.11.8 kernel.
I’m working pandas, pysam and other python libraries, so changing the code to bash doesn’t sound appealing to me.

I used os.system and subprocess to run the commands, again this ones work fine in the terminal or from bash commands. Im expecting that the command run okay and give the output file to get data i need
Heres what im traying to do:

def netMHC(seqs:list[str], haplotypes:list[str]):
    haplotypes_str = ",".join(haplotypes)
    input_path = make_input_file(seqs, "NetMHCpan")
    
    xls_path = '../test/NetMHCpan_out.xls'  # Reemplaza esto con la ruta correcta
    os.system(f"netMHCpan -p {input_path} -a {haplotypes_str} -BA -inptype 1 -xls -xlsfile {xls_path}")

the warning
../test/net.sh: línea 3: netMHCpan: orden no encontrada.
“orden no encontrada” means order doesn’t found

And when I’m traying to use subprocess:

import subprocess
def net_chop(neo_ext, len_mer):
    input_path = make_input_file(neo_ext, "chop")
    command = f"netchop ../test/{input_path} > ../test/chop.txt"
    subprocess.run(command, shell=True)
    with open("../test/chop.txt", "r") as f:
        for l in f.readlines():
            if l.split("t")[0] == (len_mer):
                return float(l.split("t")[3])
    print("file vacio")

the warning
línea 1: netchop: orden no encontrada